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. 2012 Jul 19;8(7):e1002829. doi: 10.1371/journal.pgen.1002829

Figure 1. Histone landscape at the TGM1 promoter.

Figure 1

(A) qRT-PCR of TGM1 expression in human bladder epithelia cells (RT4), undifferentiated normal human epidermal keratinocytes (NHEK U), and differentiated normal human epidermal keratinocytes (NHEK D). (B) Schematic of the human TGM1 gene. Boxes denote exons, circle denotes predicted GRHL3 binding site, arrow denotes transcription start site, and numbers correspond to location of primer pairs. (C) GRHL3 ChIP assays in NHEK U and NHEK D cells. Numbers below the X-axis denote primers specified in B. (D–F) ChIP assays for Histone 3 Lysine 4 (H3K4) mono (1), and tri (3) methylation, H3K9 acetylation (Ac) and H3K27me3 in (D) NHEK U, (E) NHEK D, and (F) RT4 cells. Numbers below the X-axes denote primers specified in B. For ChIP assays data is mean and error bars represent SD; for mRNA expression data is mean and error bars represent SEM.