Figure 4. Schematic representation of the 2F5 wt and loop-shortened CDRH3 and approximate location of the MPER.

Left, the wt 2F5 CDRH3 apex is shown as a schematic representation based upon the mAb structure. Residues P₉₈ to A_100G are shown with each residue represented by a colored cylinder. On the right, a schematic of the length-altered version, where residues T₉₉,T₁₀₀, P_100E and I_100F , represented by the green cylinders with the discontinuous border, were deleted. The red residues, L_100A, F_100B, G_100C and V_100D, were maintained for the length alterations as described in the text. The W and Y substitutions are noted next to the original residue in the variant schematic depiction (Rosetta modeling of a variant CDRH3 was performed, but clashes with peptide binding were assessed to not be compatible with the predicted conformations). The letters a, b and c represent the locations of the second W substitutions to generate antibody variants r4W2a, b and c, respectively.