Table 1. Antibody binding kinetic constants to selected MPER probes.

	MPER peptidea			Linked peptideb			ES2 scaffoldc
Ligand	kon d	koff e	KD f	kon	koff	KD	kon	koff	KD
wt 2F5	2.3	0.7	3.1	3.6	0.7	2.2	10.1	1.3	1.3
r2	0.5	5.1	90.2	1.5	8.5	55.8	8.7	2.6	3.0
r4	0.2	8.1	382	1.0	10.5	96.8	6.6	9.3	13.9
r6	0.4	18.9	390	1.8	23.7	125	7.9	31.9	40.2
e2	0.1	8.7	463	1.3	3.5	26.9	7.4	1.7	2.3
e4	0.4	10.1	239	1.3	10.4	78.6	8.5	3.6	4.2

^a MPER peptide: EQELLELDKWASLWNWFDITKWLWYIKKKKGSKKK.

^b Linked peptide: EQELLELDKWASLGGGGSGGWNWFDITKWLWYIKKKKGSKKK.

^c ES2 scaffold: scaffold protein displaying the 2F5 gp41 epitope stabilized in the antibody-bound conformation.

^dAssociation rate (k_on) is measured in (1/Ms 10⁵).

^eDissociation rate (k_off) is measured in (1/s 10⁻³).

^fAffinity constant (KD) is measured in (M 10⁻⁹ or nM).