Figure 5. Role of IL-17A signaling in neutrophils during Tc17 mediated anti-fungal vaccine immunity.

Mice were depleted of CD4⁺ T cells, vaccinated and challenged as described in Fig. 2. A. On day 4 post-challenge, BAL fluid was collected for enumeration of the percent Gr1⁺CD11a^hi (LFA-1⁺) neutrophils by flow cytometry. Values are mean ± SD of 4–5 mice/group. Data is representative of two independent experiments. B. Lung CFUs at day 6 post-challenge. 100 µg of mAb 1A8 was used at days 0, 2 and 4 post-challenge to deplete neutrophils; ≥99% of the cells were depleted as measured by flow cytometry (data not shown). CFUs are in box and whisker plots for 6–18 mice/group. Data is representative of two independent experiments. *, p<0.05 for comparison indicated.