Table 1. Bacterial strains and plasmids used in this study.

Bacterial strains and plasmids	Relevant characteristics	Reference
Escherichia coli
BW25113	Wild type	[26]
JW3932	Arginine auxotroph, ΔargH, (argininosuccinate lyase)	[26]
JW2002	Histidine auxotroph, ΔhisD, (histidinal dehydrogenase)	[26]
JW3745	Isoleucine auxotroph, ΔilvA, (threonine deaminase)	[26]
JW5807	Leucine auxotroph, ΔleuB, (3-isopropylmalate dehydrogenase)	[26]
JW2806	Lysine auxotroph, ΔlysA, (diaminopimelate decarboxylase)	[26]
JW3910	Methionine auxotroph, ΔmetB (O-succinylhomoserine lyase)	[26]
JW2580	Phenylalanine auxotroph, ΔpheA (chorismate mutase/ prephenate dehydratase)	[26]
JW0377	Proline auxotroph, ΔproC, (pyrroline-5-carboxylate reductase)	[26]
JW0003	Threonine auxotroph, ΔthrC, (threonine synthase)	[26]
JW1254	Tryptophan auxotroph, ΔtrpC, (indole-3-glycerol phosphate synthase)	[26]
JW2581	Tyrosine auxotroph, ΔtyrA, (chorismate mutase/ prephenate dehydrogenase)	[26]
JW3087	Tryptophan overproducer, ΔtdcC, (threonine STP transporter)	[26]
JW2000	Histidin overproducer, JW2000, ΔhisL, (his operon leader peptide)	[26]
Plasmids
pGRG36	Contains Tn7 transposase gene tnsABCD	[33]
pJBA24-egfp	Contains expression cassette: PlacZ-RBSII-egfp-to-t1-egfp	[31]
pGRG36-PlacZ-egfp	tnsABCD, PlacZ-RBSII-egfp-to-t1	This study