Table 2. Coefficients of determination (R²) of calibration curves generated from amino acid-dependent growth of the different amino acid biosensors tested.

Biosensor	Deleted gene	OD600 nm (R2)	RFU (R2)
Arginine	ΔargH	0.987	0.985
Histidine	ΔhisD	0.991	0.988
Isoleucine	ΔilvA	0.996	0.994
Leucine	ΔleuB	0.987	0.992
Lysine	ΔlysA	0.985	0.994
Methionine	ΔmetB	0.996	0.999
Phenylalanine	ΔpheA	0.984	0.999
Proline	ΔproC	0.943	0.960
Threonine	ΔthrC	0.981	0.969
Tryptophan	ΔtrpC	0.952	0.986
Tyrosine	ΔtyrA	0.973	0.997

Biosensor growth was determined either turbidometrically (OD_{600 nm}) or by measuring GFP fluorescence emission (RFU).