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. 2012 Jul 19;7(7):e34893. doi: 10.1371/journal.pone.0034893

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Rouzaud-Laborde et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) TPH1 mRNA expression in cardiac tissue was defined by RT-PCR (MW: molecular weight) (left panel) and Western blot (right panel). Immunoblots showed a faint immunoreactive band in heart lysates of 129/SvJ mice but not in those from 129/SvJ TPH1 KO mice. (B) TPH1 immunostaining localized within vesicular granules in rat heart (right and upper panel). Metachromatic granules of mast cells stained with toluidine blue; magnification 400X (right panels). Few mast cells stained with toluidine blue were also present in heart of 129/SvJ mice, magnification 100X (left panel).