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(A) Enzyme activity was performed by measuring the conversion of different concentration of 5-hydroxytryptophan (5-HTP) (10–100 µM) into 5-HT in homogenates of mouse cardiac tissue. Values are means ± SEM of 5-HT concentration obtained from three different heart homogenates performed in triplicate. (B) Synthesis of 5-HT from 20 µM 5-HTP in presence or not of 50 µM of AADC inhibitor benserazide. Values are means ± SEM (n = 3 to each group) ***P<0.0001.
