Figure 1. 5hmC levels are reduced in the germinal regions of the human fetal forebrain.

Fetal brain sections were stained with hematoxylin and eosin (A-C) and evaluated for 5hmC or TET2 levels using immunohistochemistry. Sections of the forebrain germinal region stained with antibodies for 5hmC (D) showed markedly reduced levels. In contrast, periventriclular regions outside the forebrain germinal matrix (E) and in the fetal cortex (F) showed high levels of 5hmC staining. TET2 immunohistochemistry (G-I) showed a similar distribution to 5hmC staining. Original magnification was 200X for all photomicrographs, except panels A, D, and G which were 100X.