Table 2. LC-MS analysis of (apo)flavodoxin.
Species | Label in Figure 8 | Expected mass (Da) | Measured mass (Da) | Δ(mass) (Da) |
Apoflavodoxin | M | 19531.8 | 19533±1.5 | +1.2 Da |
Apoflavodoxin +1 O | MO | 19547.8 | Not observed | - |
Apoflavodoxin +2 O | MO2 | 19563.8 | 19565±2 | +1.2 Da |
Apoflavodoxin +3 O | MO3 | 19579.8 | 19582±2 | +2.2 Da |
Apoflavodoxin + NBD | M-NBD | 19695.9 | 19697±2 | +1.1 Da |
Apoflavodoxin +1 O + NBD | MO-NBD | 19711.9 | 19713±2 | +1.1 Da |
Apoflavodoxin dimer | D | 39063.6 | 39066±5 | +2.4 Da |
Monomeric protein species are labelled M. Molecular masses of NBD-Cl and NBD are 199.6 and 164.1 Da, respectively. Expected mass and measured mass are average masses.