Figure 4. AGE-modification of a fibronectin substrate accompanies photooxidation/photodegradation of intracellular A2E.

(A) A2E content of ARPE19 is diminished by irradiation (430 nm) in the presence/absence of aminoguanidine (AG). UPLC quantitation; Mean ± SEM of 3 experiments. (B) AGE-modification of fibronectin substrate underlying irradiated A2E-containing ARPE19 cells. Prior accumulation of aminoguanidine within the cells reduced AGE-formation. ELISA quantitation as AGE-BSA equivalent units. The anti-AGE antibody recognized both carboxymethyllysine (CML)-BSA and carboxyethyllysine (CEL)-BSA. + presence of condition. Mean ± SEM of 7 experiments.