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. 2012 Jul 19;7(7):e41144. doi: 10.1371/journal.pone.0041144

Figure 3. GLA boosts gp140 specific B cell numbers in spleen and female genital tract.

Figure 3

Mice were immunized i.n. three times at 3 week intervals with 10 µg gp140 alone, with 10 µg GLA or 100 µg chitosan. Three weeks after the third immunization spleens were removed and gp140 specific IgA (A) and IgG (B) antibody secreting cell (ASC) numbers assessed by ELISPOT on splenocytes stimulated for 3 days with IL-2 plus pokeweed mitogen. Bars represent mean of n = 5 mice ± SEM, *p<0.05, **p<0.01, ***p<0.001. gp140 specific IgA (C) and IgG (D) antibody secreting cell (ASC) numbers assessed by ELISPOT on cells from female genital tract immediately after isolation. Bars represent mean of 2 pools of female genital tract cells, cells were pooled from either 2 or 3 mice.