Figure 4. GLA boosts T cell responses and switches to a Th17 response.

Mice were immunized 3 times with 10 µg gp140 alone or with 10 µg GLA or 100 µg chitosan. T cell proliferation responses in spleens were measured by [³H] Thymidine incorporation (A) or CFSE dilution (B) after the third immunization. Antigen specific IgG subtype responses were measured in sera (C). IL-17 (D), IFNγ (E) and IL-4 (F) were analyzed in supernatants from T cell proliferation assays by Luminex. Antigen specific T cell cytokine responses were measured by intracellular staining of gp140-peptide stimulated T cells (G). Bars represent mean of n = 5 mice ± SEM, *p<0.05, **p<0.01, ***p<0.001.