Figure 5. The fraction of SLTxA1(N⁻) that recovers activity in the cytosol is dislocated by the Hrd1 complex.

A. Ten-fold dilutions of WT (BY1747) and derivative strains null for genes associated with ER dislocation were transformed with a plasmid expressing SLTxA1(N⁻) and applied to inducing (d/o galactose) and non-inducing (d/o glucose) plates and grown for 3 d. B. Wt, ubc6, ubc7 and ubc6–ubc7 expressing SLTxA1(N⁻) strains were diluted as in A and grown for the indicated times.