Figure 3. Mitochondrial [Ca²⁺] follows the increase in cytosolic [Ca²⁺] with a delay.

A: Colocalisation of 2mt8RP and Mitotracker Orange in a β-cell, 24 h post infection. B: The effect of 17 mM glucose on V_m, [Ca²⁺]_cyt (Fura-Red) and [Ca²⁺]_mit (2mt8RP) in a single pancreatic β-cell (representative of n = 10 cells). Inset: Pseudo-colour images of the patched cell cluster presenting pixel-to-pixel ratios at the time points indicated by arrows (i – iii). ROI is indicated with red oval. C: Mean times of maximal increase for [Ca²⁺]_cyt and [Ca²⁺]_mit in pancreatic β-cells, in response to 17 mM glucose (n = 10 cells). The times are calculated from the moment of the arrival of the first action potential. *Differences are statistically significant (p<0.01).