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. 2012 Jul 19;7(7):e40845. doi: 10.1371/journal.pone.0040845

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Kuznetsova et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Concentration of ANS bound to proteins (C_b) as a superposition of the concentrations of the dye bound to the sites of mode 1 (C_b1) and mode 2 (C_b2). (b) The dependence on C_b1, C_b2. In the panel experimental data, best-fit curve and the values of molar extinction coefficients ε_b1 and ε_b2 obtained by multiple nonlinear regression are presented. (c) Absorption spectra of ANS bound to the sites of mode 1 and mode 2 in the units of the molar extinction coefficient.

Inline graphic — (a) Concentration of ANS bound to proteins (C_b) as a superposition of the concentrations of the dye bound to the sites of mode 1 (C_b1) and mode 2 (C_b2). (b) The dependence on C_b1, C_b2. In the panel experimental data, best-fit curve and the values of molar extinction coefficients ε_b1 and ε_b2 obtained by multiple nonlinear regression are presented. (c) Absorption spectra of ANS bound to the sites of mode 1 and mode 2 in the units of the molar extinction coefficient.