Figure 1. Design of microarray experiment adopting duplicate sets of loop-design microarray experiments.

Each experiment contained ten hybridizations and five experimental conditions, two controls and three treatments. Each mRNA sample was a combination of the mRNA of three donors, performed for biological replication in the loop-design microarray experiment. In addition, we utilized duplicate samples in each experiment for technical replication as well as internal control, estimate technological error and enhance the reliability of data.