Table 7. General health, neurological reflexes, and pain sensitivity.

Genotype	+/+	+/−	−/−	Sig. Level
	N = 17	N = 16	N = 15
Physical Characteristics
Fur condition (3 pt scale)	3	3	3	NS
Bald patches (%)	5.9	6.3	6.7	NS
Missing whiskers (%)	17.7	6.3	40	NS
Piloerection (%)	0	0	0	NS
Body tone (3 pt scale)	2.8+0.10	2.9+0.09	2.7+0.12	NS
Limb tone (3 pt scale)	3	3	3	NS
Skin color (3 pt scale)	3	3	3	NS
Physical abnormalities (%)	0	0	0	NS
Body weight, males (g)	26.5+1.0	26.8+0.76	26.2+0.93	NS
Body weight, females (g)	20.4+0.63	20.9+0.62	18.6+0.79	NS
Body Temperature (°C)	36.1+0.23	35.2+0.22	35.5+0.26	NS
Empty cage behavior
Transfer freezing (%)	0	0	0	NS
Wild running (%)	0	0	0	NS
Stereotypy (3 pt scale)	0	0	0	NS
Self-Grooming (3 pt scale)	0.71+0.17	1.00+0.20	1.20+0.22	NS
Exploration (3 pt scale)	2.7+0.17	2.6+0.16	2.9+0.10	NS
Motoric abilities
Trunk curl (3 pt scale)	2.0+0.15	2.3+0.16	2.3+0.11	NS
Wire hang (latency sec)	59.9+0.12	59.9+0.13	58.5+1.53	NS
Reflexes
Forepaw reach (%)	100	100	100	NS
Righting reflex (%)	100	100	100	NS
Corneal (%)	100	100	100	NS
Ear twitch (%)	100	100	100	NS
Whisker twitch (%)	100	100	90	NS
Reactivity
Auditory Startle (%)	100	100	100	NS
Struggle/Vocalizations (%)	41.2	25.0	53.3	NS
Dowel Biting (3 pt scale)	0.65+0.17	0.38+0.16	0.33+0.16	NS
Pain Sensitivity
Hot plate (latency sec)	5.4+0.57	5.4+0.42	6.2+0.48	NS
Tail flick (latency sec)	4.2+0.53	2.9+0.30	3.5+0.54	NS

Normal general health and pain responses in Cohort 1 of En2 mice. No genotype differences were detected using a standard battery of parameters. Data shown are means ± standard error of the mean (SEM) for body weight, temperature, wire hang latency, hot plate and tail flick latency, and behaviors assessed using a 3 point ranking scale. Percentage of mice that exhibited a specific neurological reflex or physical abnormality is expressed as percent of total mice within each genotype. N = 17+/+, N = 16+/−, N = 15−/−. Similar results were obtained for Cohort 2 (data not shown).