Table 1. Hematological profile of peripheral blood from mice after sensitization with vaccine adjuvants: saponin (SAP), incomplete Freund’s adjuvant (IFA), and monophosphoryl lipid A (MPL) at 1, 12, 24, 48, 96, 168, and 336 h after stimulation.
| Groups/Adjuvants | Time (h) | |||||||
| 1 | 12 | 24 | 48 | 96 | 168 | 336 | ||
| C | Total Leukocytes | 6900±244.9 | 5520±2008 | 6900±3037 | 6660±1662 | 6720±440.9 | 3300±924.7 | 4080±1134 |
| Neutrophils | 1022±286.5 | 696.8±352.3 | 837.0±194.3 | 396.0±218.3 | 776.4±211.8 | 391.2±107.9 | 816.6±353.3 | |
| Monocytes | 69.0±2.4 | 55.2±20.1 | 105.0±79.60 | 79.8±33.6 | 92.4±33.8 | 38.2±7.5 | 58.20±32.97 | |
| Lymphocytes | 5810±230.2 | 4590±1746 | 5958±2867 | 6184±1488 | 5851±805.5 | 2852±918.7 | 3196±831.1 | |
| SAP | Total Leukocytes | 3300±793.7 a,c | 6000±1897 | 10350±900 c,d | 9000±2728 d | 9525±5588 d | 9075±2765 | 9750±3969 a |
| Neutrophils | 757.5±430.3 | 3714±1368 a,c | 3740±1679 a,c,d | 3018±2044 a,c,d | 1750±1358 | 1331±557.2 | 1661±1378 | |
| Monocytes | 39.7±14.9 | 70.5±17.2 | 103.5±9. | 178.5±48.6 d | 116.3±65.2 | 111.0±43.3 | 145.0±31.2 a | |
| Lymphocytes | 2068±721.5 a,c,d | 2216±638.2 | 6507±1090 | 5804±1983 | 7703±4269 d | 7603±2439 | 7898±3749 a | |
| IFA | Total Leukocytes | 6420±962.8 | 3300±2001 | 5940±712.5 | 6360±1635 | 8160±1332 d | 11100±2741 a | 9840±1297 a |
| Neutrophils | 690.0±323.9 | 1144±696.9 | 732.0±324.5 | 827.4±341.3 | 563.4±86.7 | 1413±388.5 a | 1093±330.4 | |
| Monocytes | 93.0±69.2 | 43.5±14.8 | 103.2±27.9 | 114.0±76.5 | 117.0±56.1 | 135.6±67.2 a | 147.0±43.4 a,d | |
| Lymphocytes | 5637±666.4 | 2011±1578 a | 5093±1439 | 5419±1506 | 7480±1357 d | 9551±2792 a | 8612±1282 a | |
| MPL | Total Leukocytes | 4800±1358 a | 5460±1090 | 6180±1301 | 4300±458.3 | 2650±854.4 | 7150±5123 | 6900±3050 |
| Neutrophils | 1384±795.4 | 2428±518.4 a | 1391±331.7 | 699.0±366.8 | 760.5±228.3 | 987.0±770.5 | 1179±664.8 | |
| Monocytes | 88.8±51.4 | 73.8±22.5 | 105.8±97.6 | 72.0±42.2 | 65.0±19.4 | 83.0±48.7 | 75.7±18.8 | |
| Lymphocytes | 3317±573.8 a,c | 2958±717.3 | 4704±1319 | 3009±891.9 a | 1811±907.4 | 6080±4394 | 5623±2603 | |
The control group (C) was inoculated with 0.9% sterile saline. The absolute numbers (mm3) of total leukocytes and their subsets (neutrophils, monocytes, and lymphocytes) are expressed as mean ± standard deviation from groups of five animals/evaluation time. Significant differences (p<0.05) between groups are represented by the letters a, b, c, and d, referring to the C, SAP, IFA, and MPL groups, respectively.