Figure 4.

Histological findings reveal that Gucy2f knockdown leads to retinal dystrophy. (a) First row: overall view of the retina demonstrates abundant zpr1 staining in wild-type larvae, absent in Gucy2f_I2E3MO. (b) Left column: representative sections ( × 400) following zpr-1 antibody staining of double cones reveals (brown) double-cone staining (asterix) in the wild type in contrast to reduced staining in Gucy2f_ATGMO and in Gucy2f_I2E3MO larvae. Right column: light microscopy, hematoxylin–eosin stain (H&E, × 100. Wild-type zebrafish larvae reveal normal retinal structures and development of the photoreceptor layers. Black bracket indicates length of photoreceptor outer segments. Gucy2f_I2E3MO larvae display shortening of photoreceptor outer segments (no bracket). In the MO control, note the normal outer segments (black brackets).