Fig. 5.

Expression analysis by in situ hybridisation of mouse and human ACP. Mouse pituitaries at 18.5 dpc. a–f Expression of Bmp/BMP members 4, 2 and 7 is up-regulated in pre-tumoral mouse pituitaries at 18.5 dpc and human ACP (arrows). In the ACP mouse model pituitary, expression is mainly restricted to cell clusters. In human ACP, expression is up-regulated in cell clusters, but other tumour cells express BMP2 and BMP7. g, h Ctnnb1 and Fgf4 expression in the ACP mouse model pre-tumoral pituitary is up-regulated in cluster cells (arrows). i, j Fgf3/FGF3 expression is also up-regulated in cluster cells in both mouse and human ACP (arrows). k, l Cxcr4 expression is detected in clusters in the ACP mouse model pituitary (arrows), but it is more widely expressed in human ACP. Scale bars a, c, e, g, h, i, k, 150 μm; b, d, f, j, l 50 μm