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Determination of k2 by titration of the dark time between the first and second flashes used to photoassemble the apo-WOC. (A) Flash sequence used in this experiment. (B) Baseline corrected O2 data. (C) Integrated O2 signal (from B) for flashes 3–8 as a function of the dark time length. (Refer to Fig. 4 for sample.)
