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Photoassembly of the apo-WOC using the optimal laser flash sequence. (A) Flash sequence showing expected positions for first O2 release in fully assembled (intact) PSII (photo-assembly LO and HO models from Fig. 1). (B) Comparison of the photoassembly kinetics using the standard flash sequence (60 μs laser flashes spaced by 3 s) or the optimal flash sequence with long preflash (see panel A). The data shown correspond to the integrated O2 signal/flash.
