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. 2012 Jul 18;103(2):L17–L19. doi: 10.1016/j.bpj.2012.06.019

Figure 2.

Figure 2

Mobility changes of pMHCs upon binding to the TCR. (A) The diffusion constants of (Cy5-p)MHCs were determined in the lipid bilayer outside (solid) and within the interface between the lipid bilayer and the T cell (shaded) for the peptides MCC, T102S, and β2m. σMCC = σT102S = 30 molecules/μm2; σβ2m = 15 molecules/μm2. (B) The diffusion constant of (Cy5-MCC)MHC was obtained in the IS for various surface densities (error bars smaller than marker size). (Dashed line) Fit to Eq. S1 in the Supporting Material, assuming Dfree = 0.69 μm2/s, Dbound = 0 μm2/s, and σTCR-total = 40 molecules/μm2, yielding KD = 35 molecules/μm2.