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. 2012 Jul 18;103(2):303–312. doi: 10.1016/j.bpj.2012.05.041

Figure 3.

Figure 3

Normalized c(s20,w) distribution plots for βLg A over a pH range of 2.5–7.5. The figure shows the distribution plots for βLg A obtained from the fitting of SV data using a continuous c(s) distribution model. The three protein concentrations explored were ∼5 μM (solid line), 15 μM (dashed line), and 45 μM (dotted line); actual concentrations are given in parentheses. In 20 mM citrate and 100 mM NaCl: (A) pH 2.5 (5.3, 15.7, 33.4 μM), (B) pH 3.5 (4.8, 15.8, 26.7 μM), (C) pH 4.5 (2.5, 14.5, 43.9 μM), and (D) pH 5.5 (6.5, 16.8, 47.3 μM). In 20 mM MOPS and 100 mM NaCl: (E) pH 6.5 (3.5, 11.0, 33.0 μM) and (F) pH 7.5 (5.15, 22.6, 36.5 μM). Statistics for the fits can be found in Fig. S1, Fig. S2, Fig. S3, Fig. S4, Fig. S5, Fig. S6, and Fig. S7.