Table 1.
Equilibrium∗ and rate† constants calculated for βLg A and βLg B dimer dissociation at different pH values and buffer solutions, and an ionic strength of 100 mM NaCl from global fits‡ of SE and SV data
|
KD2-1 (/μM) |
koff (/s−1) |
kon (calc.) (/M−1 s−1) |
||||
|---|---|---|---|---|---|---|
| βLg A | βLg B | βLg A | βLg B | βLg A | βLg B | |
| pH 2.5 | 14.8 | 8.2 | 0.008 | 0.009 | 540 | 1100 |
| Citrate | (11.2–18.4) | (5.8–11.1) | (0.002–0.019) | (0.003–0.029) | ||
| pH 3.5 | 4.0 | 1.4 | [0.007]§ | [0.041]§ | —§ | —§ |
| Citrate | (2.3–6.0) | (1.1–4.5) | ||||
| pH 6.5 | 4.0 | 2.5 | Fast (> 0.1) | Fast (> 0.1) | >25,000 | >40,000 |
| MOPS | (2.7–5.6) | (1.0–7.1) | ||||
| pH 7.5 | 10.8 | 8.6 | Fast (> 0.1) | Fast (> 0.1) | >9,200 | >12,000 |
| MOPS | (7.6–15.8) | (6.3–10.9) | ||||
∗
Calculated from global fitting of SV and SE data. Values calculated from global fitting of SE or SV data are provided in Table S7.
†
Calculated from global fitting of SV data (SE data contain no kinetic signal). Values calculated from global fitting of SE and SV data are provided in Table S7.
‡
Calculated error ranges representing an estimated 69% confidence interval are reported in parentheses.
§
Indicative value, because no error range could be determined; kon not calculated.