Fig. 1.

DiMNF represses the IL1β-mediated induction of CD55 expression in Huh7 and Hep3B hepatoma cell lines. Huh7 or Hep3B cells were treated for 1 h with vehicle or 10 μM DiMNF and subsequently treated for 24 h with 10 ng/ml of IL1β. A and B, CD55 mRNA expression in Huh7 (A) and Hep3B (B) cells was measured by quantitative real-time PCR. Data represent mean mRNA expression level S.E.M. fold induction normalized to constitutively expressed ribosomal L13 mRNA. C, protein expression analysis of CD55 after treatment of Huh7 cells with 10 μM DiMNF for 1 h followed by treatment with 10 ng/ml of IL1β for 24 h. β-Actin was used as a control. D, protein was quantified by using a gamma counter and normalized to β-actin. Statistical significance is indicated: ***, p < 0.001; *, p < 0.05.