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. 2012 Aug;342(2):345–355. doi: 10.1124/jpet.112.193482

Fig. 5.

Fig. 5.

Effect of DiMNF on forskolin-mediated induction of CD55 expression. A and B, Huh7 cells were transfected with either the −858/+71 CD55 promoter (A) or the CD55 promoter with a point mutation in a CRE binding site (−71) (B) in the pGL3 basic reporter vector by using the GenePorter 3000 transfection reagent. Twenty-four hours after transfection, cells were treated with either vehicle or 10 μM DiMNF followed by 24-h treatment with 1 μM forskolin. Luciferase reporter activity was measured and normalized to β-galactosidase activity. Data represent mean ± S.E.M. fold induction relative to control. C, Hep3B cells targeted with either control or RelA/p65-specific siRNA were incubated with vehicle or 10 μM DiMNF for 1 h before treatment with 1 μM forskolin for 24 h, and CD55 mRNA expression was measured by quantitative real-time PCR. Data represent mean mRNA expression level ± S.E.M. normalized to constitutively expressed ribosomal L13 mRNA. Statistical significance is indicated: ***, p < 0.001; **, p < 0.01.