Fig. 7.

[¹²⁵I]C3b deposition on the surface of Huh7 cells. Huh7 cells were cultured in 24-well places and pretreated for 1 h with either vehicle or 10 μM DiMNF before treatment with 10 ng/ml of IL1β for 24 h. Cells were then incubated in media containing either 5% BSA, 5% normal human serum, or 5% heat-inactivated normal human serum for 30 min followed by administration of [¹²⁵I]C3. [¹²⁵I]C3b deposition was measured on the surface of cells by a gamma counter and normalized to protein. Data represent mean ± S.E.M. Statistical significance is indicated: *, p < 0.05.