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. 2012 Aug;82(2):333–343. doi: 10.1124/mol.112.078162

Fig. 1.

Fig. 1.

Inhibition of both CaMKII and calcineurin prevents morphine-induced spine collapse. A, representative live images of GFP-labeled cultured hippocampal neurons first taken at 21 DIV and then imaged again 1 and 3 days after drug treatment. Scale bar, 10 μm. B, zoomed image of a collapsing spine from the morphine-treated lane of A. Scale bar, 2.5 μm. C, quantification of spine density per 100 μm of dendrite length in neurons described in A. Morphine (Mor) treatment causes a significant decrease in spine density that is blocked by cotreatment with KN-62 (KN) or FK506 (FK). D, quantification of protrusion density of neurons described in A. Morphine treatment causes a significant decrease in protrusion density that is blocked by cotreatment with KN-62 or FK506. Repeated-measures two-way ANOVA; Bonferroni post-test: ***, p < 0.001; n = 8 to 10 neurons/group. Veh, vehicle.