Skip to main content
. 2012 Aug;82(2):333–343. doi: 10.1124/mol.112.078162

Fig. 4.

Fig. 4.

Morphine causes CaMKII translocalization from dendritic spines. A, neurons stained with anti-MOR (green in the overlay) and anti-CaMKII (red in the overlay) antibodies. MOR and CaMKII colocalize in >90% of dendritic spines. B, representative images of neurons stained with anti-PSD-95 (green in overlay) and anti-CaMKII (red in overlay) antibodies. Neurons were treated with drug at 21 DIV and then fixed at 24 DIV. Arrowheads indicate colocalization of PSD-95 and CaMKII; arrows indicate dendritic spines where CaMKII is not found. Scale bar, 10 μm. C, quantification of proportion of dendritic spines (as indicated by PSD-95) that contain CaMKII. Morphine causes the translocation of CaMKII from dendritic spines. This effect is blocked by MOR antagonists. One-way ANOVA; Bonferroni post-test: **, p < 0.01; n = 9 neurons/group. Veh, vehicle; Mor, morphine, Nal, naloxone.