Fig. 2.

EICs and MS² spectra of the AM and the AM-GSH conjugate produced by HLMs. The AM and the AM-GSH conjugate were generated through incubation at 37°C for 30 min in a reaction mixture containing 1 mg/ml HLMs, 80 μM 2-oxo-clopidogrel, and 1 mM GSH, as described under Materials and Methods. A, EICs at m/z 356.1 for the AM (trace a) and at m/z 661.0 for the AM-GSH conjugate (trace b). B, MS² spectrum of the precursor ion m/z 356.1 at 9.9 min. C, MS² spectrum of the precursor ion m/z 356.04 at 6.7 min. D, MS² spectrum of the precursor ion m/z 661.0 at 5.5 min.