Fig. 3.

Dependence on GSH of the metabolism of 2-oxo-clopidogrel by HLMs. Metabolism of 2-oxo-clopidogrel was performed in 50 mM potassium phosphate buffer (pH 7.4) with 80 μM 2-oxo-clopidogrel and varying concentrations of GSH (0–5 mM) at 37°C for 30 min, and the AM and AM-GSH conjugate levels were analyzed with ESI-LC/MS, as described under Materials and Methods. The relative amounts of the AM were calculated as the ratio of the area under the curve for m/z 356.1 to that for m/z 322.0, whereas the relative amounts of the AM-GSH conjugate were calculated as the ratio of the area for m/z 661.0 to that for m/z 322.0. ●, M3 eluting at 9.2 min; ■, M4 eluting at 9.6 min; ▴, M5 eluting at 9.9 min; ○, AM-GSH eluting at 5.5 min.