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. 2012 Aug;82(2):302–309. doi: 10.1124/mol.112.079061

Fig. 4.

Fig. 4.

Formation of the AM and the AM conjugates by HLMs in the presence of ascorbic acid, l-Cys, and NAC. The AM and the AM conjugates were formed through incubation at 37°C for 30 min in a reaction mixture containing 1 mg/ml HLMs, 80 μM 2-oxo-clopidogrel, and 1 mM concentrations of the reductants, as indicated under Materials and Methods. A, traces a, b, and c, EICs at m/z 356.1 in the presence of l-Cys, NAC, and ascorbic acid, respectively; traces d and e, EICs at m/z 475.1 and 517.1, respectively. B, MS2 spectrum of the AM-l-Cys conjugate at 5.6 min. C, MS2 spectrum of the AM-NAC conjugate at 6.3 min.