TABLE 1.

Summary of apparent K_m and V_max values for metabolism of 2-oxo-clopidogrel in the presence of GSH, NAC, and l-Cys

The metabolic reactions were performed at 37°C for 30 min, as described under Materials and Methods.

Reductant	2-Oxo-clopidogrel Consumption			AM		AM-Thiol Conjugate
	Km	Vmax	Vmax/Km	Km	Vmax	Km	Vmax
	μM	nmol · min−1 · mg HLMs−1		μM	auc · min−1 · mg HLMs−1	μM	auc · min−1 · mg HLMs−1
GSH	75 ± 8	0.40 ± 0.07	0.0053	13 ± 2	0.92 ± 0.02	46 ± 5	0.41 ± 0.05
l-Cys	64 ± 5	0.35 ± 0.04	0.0055	18 ± 3	0.88 ± 0.01	66 ± 7	0.95 ± 0.02
NAC	72 ± 4	0.43 ± 0.03	0.0059	14 ± 1	0.12 ± 0.01	50 ± 4	1.6 ± 0.09

auc, area under the curve.