TABLE 3.
Mutation studies of GluN1 and GluN2A
Fitted IC50 values were determined from composite concentration-effect curves constructed from recordings in 8 to 41 oocytes (n) as described under Materials and Methods at a holding potential of −40 mV; steady-state response was fixed at 0 for all conditions except GluN2B wild type. Data for wild-type GluN2B from Table 2 are included here for comparison.
| IC50 | Hill Slope | IC50mut/ IC50 WT | n | |
|---|---|---|---|---|
| μM | ||||
| GluN1/GluN2B | 0.10 | 1.0 | 41 | |
| GluN1(R115A)/GluN2B | 0.28 | 0.9 | 2.9 | 10 |
| GluN1(R323A)/GluN2B | 0.26 | 1.0 | 2.6 | 12 |
| GluN1/GluN2A | 47 | 1.2 | 9 | |
| GluN1/GluN2A(R181E) | 43 | 1.4 | 0.91 | 9 |
| GluN1/GluN2A(T208S) | 74 | 1.3 | 1.6 | 8 |
| GluN1/GluN2A(Q336R) | 58 | 1.1 | 1.2 | 14 |
| GluN1/GluN2A(S232T) | 44 | 1.0 | 0.93 | 10 |