Figure 4. Isotype specific antibody response of mice immunized with purified recombinant Li-ntf2 formulated with the adjuvant MPLA-SE.

Ten mice were immunized three times (three weeks apart) with saline plus the adjuvant BpMPLA-SE (A), or with the Li-ntf2 in saline (B), or with Li-ntf2 mixed with BpMPLA-SE (C). Animals were bled 10 days after the last immunization and antibody responses of IgG1 and IgG2a isotypes were measured by ELISA using biotinylated isotype rat mAbs specific for mouse IgG isotypes. The curves represent the responses of each individual mouse.