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. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: J Med Chem. 2012 Jun 22;55(13):6021–6032. doi: 10.1021/jm300253q

Figure 5.

Figure 5

(A) Inhibition of HIV-1 transcription by 5a: the CEM cells were infected with pseudo-typed HIV-1 virus expressing the firefly luciferase reporter. After 48 h post-infection, indicated amount of PNA-aminoglucosamine was supplemented in the culture medium. After 76 h, cells were harvested and assayed for firefly luciferase activity in the cell extract. The results are expressed as mean value of two independent experiments with error bar. (B) Effect of 5a on cellular proliferation: CEM cells were grown in the absence or presence of 0.1 mM and 0.5 mM of 5a dissolved in DMSO. The final concentration of DMSO supplemented through 5a in the medium was 5%. The culture medium was supplemented with 5 µCi of [methyl-3H]thymidine/mL. After 48 h, cells were harvested and [3H]-thymidine incorporated into the DNA was determined. The results expressed as percent of thymidine incorporation with respect to the control are the averages of three sets of independent experiments.