Table 3.

Splicing of DmNa_v in the aCC motoneuron in WT, sda, and phenytoin-fed sda backgrounds

Genotype/drug	DmNav	J	I	A	B	C	D	E	F	H	K	L	# Clones	%
WT	33		I	A	B		D					L	19	40.4
	58		I	A	B		D				K		9	19.1
	30		I	A	B		D		F			L	7	14.9
	54		I	A	B		D		F		K		5	10.6
	55		I	A	B		D			H		L	4	8.5
	32		I	A	B		D		F	H		L	3	6.4
sda	31		I		B		D		F			L	13	22.8
	43		I		B		D					L	11	19.3
	55		I	A	B		D			H		L	9	15.8
	33		I	A	B		D					L	7	12.3
	69		I	A	B		D				K	L	7	12.3
	30		I	A	B		D		F			L	5	8.8
	59		I	A			D					L	3	5.3
	58		I	A	B		D				K		2	3.5
sda + phenytoin	30		I	A	B		D		F			L	12	23.5
	31		I		B		D		F			L	7	13.7
	33		I	A	B		D					L	7	13.7
	58		I	A	B		D				K		7	13.7
	32		I	A	B		D		F	H		L	4	7.8
	43		I		B		D					L	4	7.8
	54		I	A	B		D		F		K		3	5.9
	55		I	A	B		D			H		L	3	5.9
	34		I		B		D		F	H		L	2	3.9
	60		I		B		D				K		2	3.9

The inclusion of exon L increases from 70.2% in WT to 96% in sda (p ≤ 0.01). Exposure of sda larvae to phenytoin rescues the inclusion of L to 76.5% (p ≤ 0.05). Note that DmNa_v69 isolated from sda contains both exons K and L. Nomenclature of DmNa_v splice variants has been described previously (Lin et al., 2009). Only those splice variants present in two or more copies are shown isolated from 50, 59, and 56 clones, respectively. DmNa_vs shown in bold (i.e., 69) are nonfunctional in that they do not give rise to functional channels. These clones are shown for reference only and have not been used for determination of frequency of inclusion for either exons K and L. The letters J–L denote alternative exons in DmNa_v, and the presence of the letter denotes inclusion in the transcript (for details, see Lin et al., 2009).