Quenched time points (0–20 minutes) were analyzed for FXIII activation in CTI whole blood from a healthy individual (panel A) and a hemophilia A individual (panel B). The polyclonal antibody used is specific for the unactivated FXIII, Mr=75,000 and activated FXIII species (FXIIIAa, Mr=71,000). Clot time (CT, arrow) in the healthy individual was 3.2 min and 9.8 minutes in the hemophilia individual. C) Quantitation of the appearance of the activated form of FXIII is compared between hemophilia (◇) and healthy (◆) whole blood. The mean clot time (CT) is shown below the graph with an arrow.