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. Author manuscript; available in PMC: 2012 Jul 20.
Published in final edited form as: Mucosal Immunol. 2008 Mar 5;1(3):219–228. doi: 10.1038/mi.2008.6

Figure 4.

Figure 4

Flow cytometry and in situ detection of Mamu A*01/gag tetramer + CD8 + T cells in the genital tract and systemic tissues 6 months after SHIV89.6 immunization. (a) Flow cytometry plots showing the Mamu-A*01 Gag181–189 CM9 and Tat28–35 SL8 tetramer + CD8 + T-cell frequencies in freshly isolated vaginal mononuclear cells and cryopreserved PBMC and axillary lymph node mononuclear cells from animal 30851. (b) In situ detection of Mamu A*01/gag CM9 tetramer + CD8 + T cells in axillary lymph nodes, cervix, and vagina from animal 30851. Tetramer stain (red, inset), CD8 antibody stain (green), and tetramer and CD8 antibody double-staining cells (yellow) are shown. Arrows indicate tetramer + CD8 + cells. All images are confocal z-scans collected using a ×60 objective lens. The bar = 20 μm. SHIV, simian–human immunodeficiency virus.