Figure 5.

Gag-specific CD8 ⁺ T-cell responses in lymphoid tissues and blood of SHIV89.6-immunized rhesus macaques. SIV-specific CD8 ⁺ T-cell responses from cryopreserved PBMCs, axillary, mesenteric, and genital lymph node mononuclear cell samples are shown as a pie chart. Empty circles indicate that there was no positive response in the indicated T-cell subset in that sample. For every positive response, the frequency of positive SIV-specific T cells was normalized to 10⁵ CD3 ⁺ T cells and that value is shown in white at the center of the pie chart. Each portion of a pie chart indicates the percentage of SIV-specific T cells that responded with one, two, or three functions, and the arcs around the pie show the function or combination of functions to which the specific response corresponds (see color legend). *Number of p27-specific CD8 ⁺ T cells (per 10⁵ CD3 ⁺ T cells). IFN-γ, interferon γ; IL-2, interleukin 2; PBMC, peripheral blood mononuclear cell; SHIV, simian–human immunodeficiency virus; SIV, simian immunodeficiency virus; TNF-α, tumor necrosis factor-α.