Table 1.

Major histocompatibility complex class I haplotype and SIV-specific interferon-γ-secreting T-cell responses in SHIV89.6-immunized monkeys

		IFN-γ spot-forming cells per 106 mononuclear cellsa
Monkey	MHC class I alleles	Acute SHIVb PBMC	Necropsy PBMC	Necropsy spleen
26817	A08	76	60	65
32357	— c	146	30	225
32365	A11	Negative	Negative	Negative
29372	—	83	Negative	Negative
30886	B01	101	Negative	35
25907	B01	66	Negative	55
30616	—	210	70	Negative
30851	A01	270	165	45
30811	B01	NDd	65	Negative

IFN-γ, interferon γ; MHC, major histocompatibility complex; PBMC, peripheral blood mononuclear cell; SHIV, simian–human immunodeficiency virus; SIV, simian immunodeficiency virus.

^aA sample was considered positive if the number of IFN-γ-secreting cells per well exceeded 50 cells per 1×10⁶ mononuclear cells and if the number of positive IFN-γ spot-forming cells (SFC) was greater than the mean number of SFC found in the medium-only wells plus 2 s.d.. Data are reported as the number of IFN-γ SFC per 1×10⁶ PBMC. For reporting purposes, the background number of IFN-γ SFC observed in medium-only wells was subtracted from the number of IFN-γ SFC of SIV peptide-stimulated wells.

^bEight to nine weeks post-SHIV89.6 immunization.

^cMHC class I loci and alleles were typed by PCR. A dash (—) indicates no known alleles.

^dND indicates that a sample was missing or results were not interpretable.