Table 4.
MHC Alleles and Microsatellite Genotypes of Indian and Chinese Origin Rhesus Macaques IVAG Inoculated with SIVmac251
| Ancestral country | Animal no. | Rhesus MHC alleles | Microsatellite locus genotypea | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mamu A*01 | Mamu B*01 | D7S794b | D17S804 | D7S513 | D3S1768 | D13S765 | D11S925 | D6S291 | D6S276 | D6S1691 | D10S1412 | D18S72 | D16S403 | D8S1106 | ||
| India | 27762 | − | − | 124/128b | 118 | 189/207 | 209/221 | 216/224 | 308 | 204 | 225/235 | 197 | 157/163 | 308/330 | 168 | 164/172 |
| 28209 | + | − | 120/128 | 118 | 197/211 | 193/229 | 208/236 | 308/330 | 200/214 | 233/235 | 197/203 | 163 | 306/330 | 168 | 144/152 | |
| 28336 | − | + | 108/124 | 114/118 | 195/207 | 205/225 | 224/228 | 312/330 | 204 | 221/235 | 197/213 | 160 | 306/328 | 156/158 | 152/156 | |
| 28349 | + | + | 108 | 118 | 187/205 | 217/221 | 232/236 | 310/330 | 206 | 225 | 213/221 | 157/163 | 306/308 | 164/168 | 160/164 | |
| 28418 | + | − | 120/124 | 118 | 189/203 | 193/217 | 224/228 | 308 | 204 | 227/235 | 197 | 157/163 | 306 | 156/174 | 140/144 | |
| 28549 | − | − | 124 | 118 | 187/219 | 205/229 | 212/224 | 308/312 | 206/214 | 233 | 199 | 157 | 308 | 164 | 144/152 | |
| 27307 | − | + | 120/124 | 118 | 195/207 | 213 | 224/232 | 312/330 | 204/206 | 215/227 | 177/197 | 157/163 | 310/324 | 152/168 | 160 | |
| 29058 | − | + | 128 | 118 | 197/203 | 225 | 208/224 | 312/338 | 206/214 | 225/231 | 197/209 | 157 | 306 | 164/174 | 144/152 | |
| 31167 | − | − | 128 | 118 | 187/189 | 201/213 | 224/236 | 312/342 | 206/214 | 225/235 | 197 | 157/163 | 306/308 | 164/168 | 144/160 | |
| 31168 | − | + | 120/128 | 118 | 191/203 | 213/221 | 216/232 | 308/338 | 206/208 | 221/235 | 197/215 | 157/166 | 306/308 | 156/158 | 148/168 | |
| 22162 | − | − | 108/124 | 118 | 203/207 | 205/221 | 208/232 | 308/338 | 206/214 | 205/221 | 197/203 | 157/160 | 306/314 | 164/170 | 144/172 | |
| 22237 | − | − | 108/128 | 118 | 195/207 | 201/205 | 212/220 | 312/330 | 204 | 227/235 | 195/197 | 157/160 | 302/328 | 144/164 | 132/144 | |
| 22990 | − | − | 128 | 118 | 187/207 | 201/225 | 224/228 | 330 | 206/212 | 225/233 | 191/197 | 157/163 | 308/330 | 152/164 | 144/168 | |
| 23657 | − | + | 128/136 | 118 | 187/207 | 225/229 | 224 | 338 | 206/214 | 233 | 199/211 | 157 | 306/308 | 164/168 | 148 | |
| 23737 | − | + | 124/128 | 118 | 219/233 | 201/225 | 232/260 | 308/338 | 204/206 | 235/237 | 195/201 | 157/160 | 306/308 | 156/168 | 152/156 | |
| 24185 | − | − | 108/128 | 118 | 205/211 | 205/217 | 228/260 | 308/312 | 204 | 221/225 | 205/213 | 157/163 | 306/328 | 164 | 144/160 | |
| 3/16 | 7/16 | |||||||||||||||
| No. of allelesc | 5 | 2 | 11 | 9 | 9 | 6 | 6 | 9 | 14 | 4 | 8 | 8 | 10 | |||
| Most common alleled (freq.) | 128 (13/32) 0.41 | 118 (31/32) 0.97 | 207 (7/32) 0.22 | 225 (6/32) 0.13 | 224 (10/32) 0.31 | 308 (10/32) 0.31 | 204 (12/32) 0.38 | 235 (8/32) 0.25 | 197 (14/32) 0.44 | 157 (17/32) 0.53 | 306 (13/32) 0.41 | 164 (11/32) 0.34 | 144 (9/32) 0.28 | |||
| Freq. het.se | 11/16 0.69 | 1/16 0.06 | 16/16 1.00 | 14/16 0.88 | 15/16 0.94 | 12/16 0.75 | 10/16 0.63 | 13/16 0.81 | 12/16 0.75 | 11/16 0.69 | 13/16 0.81 | 12/16 0.75 | 14/16 0.88 | |||
| China | 31403 | − | − | 124/128b | 106/114 | 189/207 | 213/221 | 220/232 | 330/332 | 204/206 | 211/215 | 197/217 | 154/157 | 316/320 | 166 | 144/152 |
| 31404 | − | − | 112/124 | 108/118 | 207/219 | 221/225 | 220/236 | 344/350 | 204/206 | 211/227 | 197/211 | 160 | 312/314 | 158/186 | 160 | |
| 31424 | ND | ND | 124 | 118 | 177/207 | 185/189 | 224/228 | 308/312 | 202/218 | 223/237 | 211/217 | 157/160 | 312/326 | 140/152 | 144/148 | |
| 31426 | − | − | 128 | 114 | 198/297 | 205/217 | 220/228 | 328/332 | 204/206 | 225 | 209/211 | 157/163 | 316/332 | 142/156 | 156/160 | |
| 31427 | ND | ND | 128 | 120/126 | 199/217 | 197 | 228/264 | 328/330 | 204 | 231 | 177/203 | 157 | 312/316 | 156/160 | 148/160 | |
| 31428 | ND | ND | 108/128 | 118/122 | 205/207 | 209 | 224/272 | 328/330 | 208/222 | 223/227 | 177/213 | 157 | 308 | 152/166 | 148/160 | |
| 31405 | − | − | 124 | 114/122 | 195/209 | 213/221 | 220/232 | 330/332 | 204/206 | 211/215 | 197/217 | 154/157 | 316/320 | 166 | 144/152 | |
| 31406 | − | + | 124 | 114/122 | 193/199 | 185/213 | 220/224 | 328/332 | 208/216 | 211/213 | 195/213 | 160 | 312/320 | 150/172 | 160 | |
| 31409 | − | − | 124/128 | 114 | 191/207 | 201/217 | 220/224 | 308/310 | 204/206 | 211 | 201/205 | 154/163 | 306 | 158/160 | 144/156 | |
| 31410 | − | − | 108/128 | 114/120 | 199/211 | 185/229 | 232/236 | 320/324 | 204 | 223/229 | 203/205 | 154/160 | 314 | 142/158 | 152/156 | |
| 31415 | − | − | 108/128 | 114/118 | 193/197 | 193/213 | 200/224 | 322/330 | 202/214 | 211 | 207 | 157/160 | 318/324 | 142/166 | 144/160 | |
| 31433 | − | − | 124/132 | 112/122 | 199/209 | 205/221 | 264/268 | 312/348 | 202/204 | 211 | 207/209 | 157/172 | 312/336 | 158/162 | 140/160 | |
| 31436 | − | − | 128 | 108/118 | 205 | 185/201 | 224/228 | 330/332 | 200/202 | 211 | 193/199 | 157 | 310/322 | 144/166 | 144 | |
| 31437 | − | − | 124 | 116 | 189/193 | 209/217 | 228 | 312/348 | 200/204 | 223 | 203/211 | 154/157 | 312/318 | 146/162 | 148/152 | |
| 0/10 | 1/10 | |||||||||||||||
| No. of allelesc | 6 | 9 | 13 | 12 | 9 | 12 | 9 | 9 | 13 | 5 | 12 | 13 | 6 | |||
| Mostd common allele (freq.) | 124 (12/28) 0.43 | 114 (9/28) 0.32 | 207 (6/28) 0.21 | 185, 213, 221 (4/28) 0.14 | 220, 224, 228 (6/28) 0.21 | 330 (6/28) 0.21 | 204 (11/28) 0.39 | 211 (12/28) 0.43 | 211 (4/28) 0.14 | 157 (13/28) 0.46 | 312 (6/28) 0.21 | 166 (7/28) 0.25 | 160 (9/28) 0.32 | |||
| Freq. het.se | 7/14 0.50 | 10/14 0.71 | 13/14 0.93 | 11/14 0.79 | 13/14 0.93 | 14/14 1.00 | 12/14 0.86 | 7/14 0.50 | 13/14 0.93 | 9/14 0.64 | 11/14 0.79 | 12/14 0.86 | 11/14 0.79 | |||
Abbreviation: ND, Not done.
As detected with heterologous human primers.
Each locus is designated as for human microsatellite marker locus; alleles at each locus are designated by a number indicating mobility of PCR product on a polyacrylamide gel; two numbers separated by a slash (/) indicates a heterozygote; a single number indicates only one allele, which is most likely a homozygote; alternatively, a null allele could also be present.
Number of alleles observed in all animals from one ancestral country.
Allele (or alleles) that are most frequently detected in animals from one ancestral country. The number of alleles over the total number of alleles (2 × number of individuals for diploids) is given in parentheses. Animals for which a single allele was detected were inferred to be homozygous and, thus, were assumed to carry two copies of the same allele.
Frequency of heterozygous animals (i.e., two different alleles detected) at each locus defined as: the number of animals heterozygous at a locus over the total number of animals.