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. Author manuscript; available in PMC: 2012 Jul 20.
Published in final edited form as: AIDS. 1998 Jan 1;12(1):1–10. doi: 10.1097/00002030-199801000-00001

Table 1.

Virus isolation from peripheral blood mononuclear cells (PBMC) of targeted iliac lymph-node (TILN)-immunized and control rhesus macaques after intravaginal challenge with SIVmac251.

Challenge
timepoint
(week)		 Week post-challenge
Macaques Vaccine No.
vaccinations		 1 2 4 6 8 12 16 20 24 28 %
Positive*
Group A
    22575 Whole SIV 3 10 + + + + + + + + + + 100
    24331† Whole SIV 3 10 + + + + + + + 100
    25428 Whole SIV 3 10 + + + + + + + + + + 100
Group B
    21747 p27 + gp120 3 10 + + + + + + + + + + 100
    24816 p27 + gp120 3 10 − − − − − − − − − − 0
    24428† p27 + gp120 3 10 + + + + + + + + 100
Group C
    25255 None 0 10 + + + + + + + + 100
    25422 None 0 10 + + + + + + + + 100
    24803 None 0 10 + +§ 100
    25597 None 0 10 + +§ 100
Group D
    20634† Pr55 + gp130 4 17 + + − ND + + 80
    22212† Pr55 + gp130 4 17 − + + ND + + 80
    22590 Pr55 + gp130 4 17 − + + ND + + 80
Group E
    26665 ‡ None 0 17 + − − ND − − 20
    26742 None 0 17 − − + ND + + 60
*

Of the PBMC cocultures that were performed, this is the percentage from which simian immunodeficiency virus (SIV) was isolated.

†

Animals were sacrificed at 19 weeks (24331, group A), 23 weeks (24428, group B), 35 weeks (20634, group D), 41 weeks (22212, group D) and 34 weeks (26665, group E). At necropsy, PBMC and mononuclear cell suspensions obtained from spleen, inguinal, iliac and axillary lymph nodes were cocultivated with CEM-X174 cells as described for PBMC in Material and methods. All these cultures for all animals were virus isolation-positive, except control animal (26665). PBMC and mononuclear cells suspensions obtained from spleen and lymph nodes of this animal (26665) were virus isolation-negative.

‡

In addition to the standard PBMC coculture technique described in Materials and methods, CD8+ T cells were depleted from the PBMC of this animal (26665) at weeks 9 and 12 by using immunomagnetic beads (Dynal, Inc., Lake Success, New York, USA). The CD8+ T-cell-depleted PBMC were cocultured with CEM-X174 cells. Both CD8+-depleted cultures were virus isolation-negative.

§

Animals were killed at this time for experimental reasons unrelated to this study. None, No immunization; ND, not done.