Figure 3. Dynamic MTs are required for the initiation of NWS virus infection in LLC-MK2 cells.

LLC-MK2 cells were either untreated (NT) or kept in E-MEM plus TAX or Na₃VO₄ for 30 min and then infected for 5 h with NWS virus (m.o.i. = 0.1 p.f.u./cell) in the absence (NT) or presence of drugs. TAX and Na₃VO₄ were kept in the culture media for 2 h p.i. and then cell monolayers were incubated in drug-free media for the residual infection time. The graphic represents the percentage of NWS infected cells, according to NP labelling by IIF. Each sample was processed in duplicate. Mean values from three independent experiments were analyzed. Error bars correspond to standard deviations.