Figure 3. Posttranslational modifications for bovine BST-2s.

(A) Expression plasmid for FLAG-tagged bBST-2A1, bBST-2A2 or bBST-2B was transfected into HEK 293T cells. Cells were dissolved 24 h after transfection and subjected to 12.5% SDS-PAGE. Western blotting was performed with anti-FLAG or anti-Actin antibody. (B) Bovine BST-2s expressed in HEK 293T cells were treated with (+) or without (−)PNGase F for de-N-glycosylated reaction, and then subjected to 12.5% SDS-PAGE. Reaction conditions with PNGase F were as described in the Materials and Methods. BST-2 was detected with anti-FLAG antibody by Western blotting analysis.