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. 2012 Jul 20;7(7):e41481. doi: 10.1371/journal.pone.0041481

Figure 2. TEM overview of the development of Bd in skin explants of Xenopus laevis.

Figure 2

(A) adhesion of an encysted zoospore (ZS) to the superficial mucus layer (M) on top of the stratum corneum (SC); at the site where adhesion occurs the cell wall of the encysted zoospore is remarkably thickened (arrow); scale bar = 500 nm; (B) initiation of germ tube development (arrow); note the polarisation of the cell cytoplasm (*); scale bar = 2 µm; (C) germ tube (GT) elongating upon the epidermis of X. laevis, with the presence of numerous lipid globules (LG) in the germ tube; scale bar = 1 µm; (D) a growing germ tube protruding the stratum corneum; scale bar = 2 µm; (E) invasion of a host cell resulting in the loss of cell cytoplasm; remnants of the host cell cytoplasm (arrow) are seen at the tip of a protruded germ tube; note the presence of a collapsed sporangium (ZS) due to cell polarisation (*); (SS): stratum spinosum; scale bar = 2 µm; (F) infected epidermal cell with digested cell content (*) alternated by an uninfected normal epidermal cell; note the presence of lipid globules in the infected host cell; scale bar = 1 µm.