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. 2012 Jul 20;7(7):e41240. doi: 10.1371/journal.pone.0041240

Figure 2. CD8+ T cells from Mam-A2.4 DNA vaccinated HLA-A2+ transgenic mice traffic into breast tumors at a higher frequency.

Figure 2

Spleen (SP), draining lymph node (DLN) and tumor (TIL) sections from SCID-beige mice receiving splenocytes from HLA-A2+ transgenic mice vaccinated with cDNA encoding either full-length or Mam-A epitopes were analyzed by means of immunohistochemistry for the presence of CD8 T cells (A) 7, and (C) 28 days post transfer (magnification of 20×). Morphometric analysis was also performed (B) 7 and (D) 28 days post transfer. Results are expressed as the mean (±SEM) of 3 different experiments. (E–F) Spleen (SP), draining lymph node (DLN) and tumor (TIL) sections from SCID-beige mice receiving splenocytes from HLA-A2+ transgenic mice vaccinated with cDNA encoding Mam-A2.4 were analyzed for the presence of CD8 T cells on days 7, 21, 28 and 35 post spleen cell transfer. Data are representative of at least 2 experiments with at least 3 mice per group/experiment. * P<0.05, ** P<0.01 and *** P<0.0001.