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. 2012 Jul 20;7(7):e41251. doi: 10.1371/journal.pone.0041251

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Kumar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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293T cells were transfected with HA-MAGI-1 and either FLAG-H6N6 NS1 ESEA or FLAG-H6N6 NS1 ESEV plasmids. At 48 hours post-transfection cell extracts were prepared and used in immunoprecipitations with anti-HA or anti-FLAG antibody. Products of immunoprecipitations were examined in immunoblots.